Possible solution for a deadly amphibian disease may already be swimming around the amphibians
[Another version of this article first appeared on The Scientist.]
In the Pyreneans mountains between France and Spain, a team of scientists hurried ahead on a trail that snaked across the undulating landscape. They had just sampled a lake and were trekking to the next, oblivious of the beauty around them—blue skies, rocky ridges, grassy meadows. Their minds dwelled on the dwindling frog numbers in the previous lake: the frogs were sick, many had died.

Schmeller and his team studied frog populations in the Pyrenean mountains. Some scientists have better luck than others! [Credit: Schmeller]
![A hardy donkey helped transport water samples from the lakes to the lab. [Credit: Schmeller]](https://scienceilluminates.files.wordpress.com/2014/02/waterdonkeys_july2012_034.jpg?w=300&h=199)
A hardy donkey helped transport water samples from the lakes to the lab. [Credit: Schmeller]
Chytridiomycosis has been decimating amphibian populations worldwide since the 1980s. “We have no means to stop its advance,” said Antje Lauer, a microbial ecologist at California State University Bakersfield, “and no cure that can be used in the wild to protect amphibians from it.”
Bd affects the amphibian skin, disrupting its ability to regulate electrolytes in the body, explained Jamie Voyles, an infectious disease ecologist at New Mexico Tech. Infected frogs lose excessive amounts of sodium and potassium, which are critical to keep their hearts pumping. Eventually, their hearts stop.
But new research suggests a potential preventive agent against Bd infection—one that may already be swimming all around the affected amphibians. Two recent studies demonstrated that aquatic microscopic fauna—such as Daphnia, Paramecium, and rotifers—can consume free-floating Bd zoospores, thereby keeping Bd from infecting as many frogs.

Schmeller and team analysing lake water samples. [Credit: Schmeller]
Schmeller’s team found that low-Bd lake water contained more microscopic fauna than high-Bd lake water. These microfauna were mostly single-celled organisms like Paramecium and rotifers, which feed on organic matter they filter from their aquatic environments.
![Alytes obstetricans, the common midwife toad. It is very susceptible to Bd infection. [Credit: Schmeller]](https://scienceilluminates.files.wordpress.com/2014/02/schmeller13.jpg?w=300&h=199)
Alytes obstetricans, the common midwife toad. It is very susceptible to Bd infection. [Credit: Schmeller]

Bd spores marked with fluorescence stain showed up in the guts of aquatic microfauna. These predators do eat the spores! [Credit: Schmeller}
This study confirmed previous observations suggesting that aquatic microfauna might prey on Bd zoospores, and showed that these microfauna can even reduce Bd infection. Yet in the lakes where Bd and amphibians interact, aquatic microfauna can choose from a variety of foods beyond the infectious zoospores. Would microfauna still reduce Bd zoospores in a more complex environment?
Catherine Searle, a disease ecologist at University of Michigan, attempted to address that question in an Ecology and Evolution paper published this past fall (September 23). She and her colleagues sought to see whether Daphnia magna and the smaller D. dentifera—both aquatic zooplanktons that filter food in the water—could consume Bd zoospores and reduce Bd infection among tadpoles when also provided with an alternative food, algae.
Although both Daphnia species decreased Bd zoospores in the water, only D. magna appeared to help reduce Bd infection in tadpoles. Searle’s team hypothesized that the faster filtering by the larger D. magna might have damaged Bd zoospores more than the smaller D. dentifera could.
Algae clearly affected the interactions among Daphnia, Bd zoospores, and tadpoles. Searle provided D. dentifera with either high or low algae supply and examined the consequent Bd zoospore numbers and tadpole infection rates. Bd zoospores survived better in the high-algae environment but tadpole infection rates did not change. While the researchers propose several mechanisms by which algae might impede Daphnia predation of Bd zoospores, to date they remain untested.
“The effects of Daphnia on Bd are not straightforward,” explained Searle, noting that Daphnia may not always reduce chytridiomycosis. “It’s complicated.”
The works of Schmeller and Searle highlight the potential of aquatic predators to fight Bd. “Aquatic predators of fungal zoospores are exciting,” said Douglas Woodhams, a disease ecologist and conservation biologist at University of Colorado, Boulder, “because they are the one factor that may be more easily manipulated for disease management” when compared with factors like pond temperature or host immunity.
However, Searle warned that “biocontrol must be implemented with caution for it might have unpredictable effects on the community.” Schmeller agreed, suggesting that biocontrol trials “should first be done in a couple of lakes…not more than that…to see if they work.”
“The studies of Schmeller and Searle remind us that host and pathogens do not exist in a vacuum,” said Voyles, “but are parts of diverse, dynamic ecosystems with many interfering organisms, chemicals and abiotic factors.”
“These studies make clear that a healthy environment with natural predators of Bd can reduce the risk of contracting chytridiomycosis,” added Lauer.